Cartridge-based automated BCR-ABL1 mRNA quantification: solving the issues of standardization, at what cost?
نویسندگان
چکیده
منابع مشابه
Cartridge-based automated BCR-ABL1 mRNA quantification: solving the issues of standardization, at what cost?
BACKGROUND Molecular monitoring of chronic myeloid leukemia patients treated with tyrosine kinase inhibitors is essential for therapeutic stratification. Inter-laboratory reproducibility is, therefore, a crucial issue which requires standardization and strict alignment of BCR-ABL1 values to the international scale. An automated cartridge-based assay (Xpert BCR-ABL Monitor(™), Cepheid) had been ...
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An automated cartridge-based detection system (GeneXpert; Cepheid) is being widely adopted in low throughput laboratories for monitoring BCR-ABL1 transcript in chronic myelogenous leukaemia. This Australian study evaluated the longitudinal performance specific characteristics of the automated system.The automated cartridge-based system was compared prospectively with the manual qRT-PCR-based re...
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T reatment of children with BCR-ABL1– positive ALL has been revolutionized with the advent of TKI therapy. Historically, children with BCR-ABL1–positive ALL were treated with intensive chemotherapy followed by allogeneic hematopoietic stem cell transplantation (HSCT), and despite this, event-free survival rates of ,50% were observed.With the advent of TKI therapy, outcomes have improved signifi...
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متن کاملEstablishment and validation of analytical reference panels for the standardization of quantitative BCR-ABL1 measurements on the international scale.
BACKGROUND Current guidelines for managing Philadelphia-positive chronic myeloid leukemia include monitoring the expression of the BCR-ABL1 (breakpoint cluster region/c-abl oncogene 1, non-receptor tyrosine kinase) fusion gene by quantitative reverse-transcription PCR (RT-qPCR). Our goal was to establish and validate reference panels to mitigate the interlaboratory imprecision of quantitative B...
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ژورنال
عنوان ژورنال: Haematologica
سال: 2011
ISSN: 0390-6078,1592-8721
DOI: 10.3324/haematol.2010.034389